OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA)

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA)
Author :
Publisher : OECD Publishing
Total Pages : 42
Release :
ISBN-10 : 9789264916784
ISBN-13 : 9264916784
Rating : 4/5 (784 Downloads)

Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA) by : OECD

Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA) written by OECD and published by OECD Publishing. This book was released on 2019-06-18 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of the test is to measure the capacity of a chemical to activate or inhibit the transcription of a genetic construct (THb/ZIP-GFP X. laevis eleutheroembryos) either by binding to the thyroid hormone receptor or by modifying the amount of thyroid hormone available for transcription. The test is based on the use of a transgenic frog (possessing the genetic construction THb/ZIP-GFP) of the species X. laevis. GFP is a fluorescent protein that can be used to affirm the presence of the substance and to follow its action. This test is based on the quantification of fluorescence, and thus of the GFP protein, throughout the eleutheroembryo exposed to a test chemical. Indeed, if the fluorescence is > 12% by including the highest concentration of the test, the test is considered positive. Organisms are exposed in chemically inert cell culture plates (6 wells). Each well contains ten organisms in solution. In a run, 20 organisms are exposed to each test concentration. Tadpoles are exposed to the test chemical at NF stage 45 for a duration of 72 hours. A positive result of the XETA test indicates in vivo that a substance induces damage to the receptor and metabolization of the thyroid hormone. A negative result indicates that, under test conditions, the test substance does not induce detectable damage to the thyroid and its functioning.


OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA) Related Books

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA)
Language: en
Pages: 42
Authors: OECD
Categories:
Type: BOOK - Published: 2019-06-18 - Publisher: OECD Publishing

DOWNLOAD EBOOK

The purpose of the test is to measure the capacity of a chemical to activate or inhibit the transcription of a genetic construct (THb/ZIP-GFP X. laevis eleuther
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 231: Amphibian Metamorphosis Assay
Language: en
Pages: 33
Authors: OECD
Categories:
Type: BOOK - Published: 2009-09-08 - Publisher: OECD Publishing

DOWNLOAD EBOOK

This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 251: Rapid Androgen Disruption Activity Reporter (RADAR) assay
Language: en
Pages: 44
Authors: OECD
Categories:
Type: BOOK - Published: 2022-06-30 - Publisher: OECD Publishing

DOWNLOAD EBOOK

The Rapid Androgen Disruption Activity Reporter (RADAR) Test Guideline describes an aquatic assay that utilizes transgenic Oryzias latipes (O. latipes, Japanese
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 210: Fish, Early-life Stage Toxicity Test
Language: en
Pages: 24
Authors: OECD
Categories:
Type: BOOK - Published: 2013-07-26 - Publisher: OECD Publishing

DOWNLOAD EBOOK

The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species t
OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA)
Language: en
Pages: 38
Authors: OECD
Categories:
Type: BOOK - Published: 2015-07-28 - Publisher: OECD Publishing

DOWNLOAD EBOOK

This Test Guideline describes the Medaka Extended One Generation Test (MEOGRT), which exposes fish over multiple generations to give data relevant to ecological