Download or read book DNA Nanostructures as Templates for Hybrid Supramolecular Assemblies written by Thomas Edwardson and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "DNA is used for the storage and propagation of genetic information in all living organisms. It is the programmability and molecular recognition properties of DNA which make this possible. Taking advantage of these properties, researchers have developed DNA self-assembly as a highly predictable method of bottom-up nanofabrication. The resulting nanostructures have the potential to solve important problems in diverse fields, from nanophotonics to gene therapy. To date most DNA nanostructures have been made purely from unmodified, natural DNA. To achieve structural complexity needed for functional devices, researchers have created intricate designs which often require a large number of DNA strands of different sequence,with increased cost and assembly error rates. This thesis examines the chemical modification of nucleic acids and their integration into DNA nanostructures. This approach is expected to simplify design and significantly reduce the number of DNA components, while adding functional complexity. Specifically, the introduction of orthogonal supramolecular interactions to DNA nanostructures and the resultant properties of these hybrid systems is investigated. Firstly, the synthesis of novel dendritic DNA amphiphiles and their self-assembly properties are investigated. The site-specific positioning of the amphiphiles on a cubic DNA scaffold allows the anisotropic organization of hydrophobic 'residues', in a manner similar to the side-chains on a protein backbone. A new set of self-assembly rules is discovered, in which these nanostructures show a geometry-dependent inter- or intra-molecular association. This is used to create the first example of a DNA nanostructure which can encapsulate and release small molecule drugs, an important challenge in nanomedicine. Secondly, this synthetic methodology is developed to produce a family of DNA-polymer conjugates, with sequence-defined polymers appended to DNA. These conjugates exhibit tunable self-assembly properties, dependent on the sequence of monomers in the polymer portion. Finally, the modular synthetic approaches developed are used to produce novel gold-binding DNA conjugates. The organization of these on prismatic DNA scaffolds creates patterns of DNA strands which can be efficiently transferred to gold nanoparticles. This template-guided 'printing' approach provides control over the number, directionality, geometry and sequence asymmetry of DNA strands bound to gold nanoparticle. Overall, the development of new synthetically modified oligonucleotides and their incorporation into nanostructures is shown to augment the field of DNA nanotechnology, introducing protein-inspired interactions and providing new tools for the creation of functional nanodevices for applications in the fields of nanophotonics, nanoelectronics and biological sensing, as well as drug and oligonucleotide delivery." --