Download or read book The Design of Oligonucleotides Which Attack Specific Gene Targets written by and published by . This book was released on 1989 with total page 19 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the first year of Navy support, we have refined our understanding of triple helix formation and in the process, have synthesized a set of triplex forming oligonucleotides directed against sites in the control region of interesting eukaryotic genes. We have shown that, in favorable instances, the dissociation constant for triplex formation can exceed 10-9M under physiological conditions. Work has begun to extend those structural analyses, by application of molecular modeling and 2D NMR methods. Work has also begun to apply photochemical analysis of triplex formation for the purpose of mapping triplex formation on gene target sites (HPRT and EGFR) within intact eukaryotic cells. Keywords: DNA recognition; Triplex helix formation.